Hypothesis The enzyme catalase, under optimum conditions, effectively speeds the decomposition of hydrogen peroxide. The Baseline Assay This part of the lab required 10 mL of 1.
In Part 2, 5 mL of catalase was placed in a test tube and put in a boiling water bath for five minutes. After mixing well, a 5 mL sample was removed and placed over a white sheet of paper. Before starting the actual experiment a lot of preparation was required. The optimum pH for an enzyme-catalyzed reaction is neutral 7 on the pH scale.
If the salt concentration is too low, the enzyme side chains will attract each other and form an inactive precipitate. In enzyme-catalyzed reactions, a substrate, the substance to be acted upon, binds to the active site on an enzyme to form the desired product.
Each active site on the enzyme is unique to the substrate it will bind with causing each to have an individual three-dimensional structure. Enzymes also have a temperature optimum, which is obtained when the enzyme is working at its fastest, and if raised any further, the enzyme would denature.
A 5-mL syringe was used to add KMnO4, 1 drop at a time until a persistent brown or pink color was obtained. Then, 1 mL of fresh catalase solution was added and the reaction was observed and recorded.
The percent of the spontaneously decomposed H2O2 was calculated. In Part 3, the materials used were 10 mL of 1. They allow these complex biochemical reactions to occur at a relatively low temperature and with less energy usage.
However, its unique protein structure under certain circumstances can easily be denatured. Hydrogen peroxide decomposes to form water and oxygen as in the following equation: In Part 3, 10mL of 1. To determine the amount of H2O2 remaining, 10 mL of 1. Enzymes require a very specific environment to be affective.
Six labeled cups were set out according to their times and 10 mL of H2O2 were added to each cup. The same steps were repeated for the 30, 60, and second cups, respectively.
The active site is the only part of the enzyme that reacts with the substrate. Some of the factors that affect enzyme reactions are salt concentration, pH, temperature, substrate and product concentration, and activators and inhibitors.
At 10 seconds, 10 mL of H2SO4 were added to stop the reaction.3. Demonstrating the presence of catalase in living tissue: a.
What do you think would happen if the potato or liver was boiled before being added to the H2O2? The Abnormal Biology of A Baby Joseph was an unhappy baby. He didn’t sleep for long periods and appeared to cry all a time.
He’d best if he had been held and rocked, or walked. View ans_pwb_3+4_e from CHEM at The Hong Kong University of Science and Technology. New Senior Secondary Mastering Biology (Second Edition) Practical Workbook for SBA 3. Sba Practical Task 3 Life Science Grade 12 sba-practical-tasklife-science-grade Life Science Practical Sba Task 3 life-science-practical-sba-task The cut (or threshold) scores for Levels 1, 2, 3, and 4, within the scale score range, were initially developed by the Smarter Balanced Assessment Consortium, with input from thousands of educators and community members.
With this momentum there's no stopping us.
The next SBA meeting is scheduled for in sunny Brisbane, Australia. Stay tuned for udpates. If you can't wait, get your fix of Syn Bio and Genome editing on 26 November for the Sc extravaganza in Sydney. Stay tuned for details.Download